Rapid Detection of Norovirus from Fecal Specimens by Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay

Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay.

The development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3' noncoding region for the rapid detection and differentiation of dengue virus serotypes are reported. The RT-LAMP assay is very simple and rapid, wherein the amplification can be obtained in 30 min u...

Rapid Detection of West Nile Virus for Loop-Mediated Isothermal Amplification Real-Time Reverse Transcription

Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus.

A one-step, single tube, real-time accelerated reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the envelope gene of West Nile (WN) virus. The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, efficiency, and rapidity under isothermal conditions with a set of six specially designed pri...

Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus

BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...

Real - Time Detection of HIV - 2 by Reverse Transcription , Loop - Mediated 1 Isothermal Amplification

Real-Time Detection of HIV-2 by Reverse Transcription, Loop-Mediated 1 Isothermal Amplification 2 3 Kelly A. Curtis*, Philip Niedzwiedz, Ae S. Youngpairoj, Donna L. Rudolph, and S. Michele 4 Owen 5 6 Laboratory Branch, Division of HIV/ AIDS Prevention, National Center for HIV/AIDS, 7 Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, 8 30333 9 10 The fin...